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mouse monoclonal santacruz biotechnology sc  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse monoclonal santacruz biotechnology sc
    Mouse Monoclonal Santacruz Biotechnology Sc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 8652 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+tubulin/%CE%B1+Tubulin+Antibody/pm42814109-32-50-52
    Average 97 stars, based on 8652 article reviews
    mouse monoclonal santacruz biotechnology sc - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Western Blot:

    Article Title: Interfacial Coordination-Engineered Emulgel Modulates the Osteo-Immune Microenvironment in Periodontitis Treatment Via HIF1 Signaling Pathway-Induced Metabolic Reprogramming.
    Article Snippet: .. Antibodies used in western blot as follows: mouse anti-MYD88 antibody (1:2000, 67969-1-Ig, Proteintech, China), mouse anti-mTOR antibody (1:25 000, 66888-1-Ig, Proteintech, China), rabbit anti-AMPK antibody, rabbit anti-HIF-1α(1:1000, ab207442, Abcam, USA), mouse anti-NF-kB (1:1000, #Q04206, Cell Signaling Technology, USA), mouse anti-TNF-α (Proteintech, 60291-1- Ig, 1:1000), rabbit anti-iNOS (1:200, 22226-1-AP, Proteintech, China), mouse anti-NLRP3 (1:2000, 68102-1-lg, Proteintech, China), rabbit antiIL-1β (1:1000, ab254360, Abcam), mouse anti-Tubulin (1:100, sc-5286, Santa Cruz Biotechnology, USA), IRF4 (1:1000, ab315394, Abcam, USA), mouse anti-STAT3 (1:1000, #9139, Cell Signaling Technology, USA), rabbit anti-GLUT1(1:1000, 21829-1-AP, Proteintech, China), rabbit anti-ERK1/2 (1:500, WL01864, Wanleibio), rabbit anti-p-ERK1/2 (1:1000, 28733-1-AP, Proteintech, China), mouse anti-AKT(1:100, sc-5298, Santa Cruz Biotechnology, USA), rabbit anti-RUNX2(1:200, 20700-1-AP, Proteintech, China), and then incubated with HRP (horseradish peroxidase) conjugated secondary antibodies, anti-rabbit (1:5000, Cat No. SA00001-2, Proteintech, China) and anti-mouse (1:5000, Cat No. SA00001-1, Proteintech, China). .. The bound antibody was detected using a standard chemiluminescence method with the ChemiDoc Touch Imaging System. (Bio-Rad, Universal Hood ll, USA).

    Incubation:

    Article Title: Interfacial Coordination-Engineered Emulgel Modulates the Osteo-Immune Microenvironment in Periodontitis Treatment Via HIF1 Signaling Pathway-Induced Metabolic Reprogramming.
    Article Snippet: .. Antibodies used in western blot as follows: mouse anti-MYD88 antibody (1:2000, 67969-1-Ig, Proteintech, China), mouse anti-mTOR antibody (1:25 000, 66888-1-Ig, Proteintech, China), rabbit anti-AMPK antibody, rabbit anti-HIF-1α(1:1000, ab207442, Abcam, USA), mouse anti-NF-kB (1:1000, #Q04206, Cell Signaling Technology, USA), mouse anti-TNF-α (Proteintech, 60291-1- Ig, 1:1000), rabbit anti-iNOS (1:200, 22226-1-AP, Proteintech, China), mouse anti-NLRP3 (1:2000, 68102-1-lg, Proteintech, China), rabbit antiIL-1β (1:1000, ab254360, Abcam), mouse anti-Tubulin (1:100, sc-5286, Santa Cruz Biotechnology, USA), IRF4 (1:1000, ab315394, Abcam, USA), mouse anti-STAT3 (1:1000, #9139, Cell Signaling Technology, USA), rabbit anti-GLUT1(1:1000, 21829-1-AP, Proteintech, China), rabbit anti-ERK1/2 (1:500, WL01864, Wanleibio), rabbit anti-p-ERK1/2 (1:1000, 28733-1-AP, Proteintech, China), mouse anti-AKT(1:100, sc-5298, Santa Cruz Biotechnology, USA), rabbit anti-RUNX2(1:200, 20700-1-AP, Proteintech, China), and then incubated with HRP (horseradish peroxidase) conjugated secondary antibodies, anti-rabbit (1:5000, Cat No. SA00001-2, Proteintech, China) and anti-mouse (1:5000, Cat No. SA00001-1, Proteintech, China). .. The bound antibody was detected using a standard chemiluminescence method with the ChemiDoc Touch Imaging System. (Bio-Rad, Universal Hood ll, USA).

    Article Title: Functional characterization of DPP4 and FcRn as receptor and coreceptor for classical human astroviruses in Caco-2 cells
    Article Snippet: Cellular lysates of siRNA-transfected cells or cell lysates of untransfected WT, FcRn-KO, DPP4-KO, or double FcRn/DPP4-KO Caco-2 cells were separated by 10% SDS-PAGE; the proteins were then transferred to nitrocellulose membranes (Millipore #HATF00010). .. The membranes were blocked with 5% nonfat milk for 1 h at RT, followed by incubation with either rabbit anti-FcRn (Abcam #ab228975) or anti-DPP4 (Cell Signaling #67138) (dil. 1:1,000) polyclonal antibodies or with mouse anti-tubulin (Santa Cruz Biotechnology #sc-51500) monoclonal antibody for 1 h at RT. .. The unbound antibody was washed three times with PBS-0.1% Tween 20, and secondary goat anti-rabbit IgG or goat anti-mouse IgG peroxidase-conjugated antibodies (ThermoFisher #31460 and #31432), diluted 1:3000 in PBS-0.1% Tween 20, were added and incubated for 1 h at RT.

    Article Title: PIWI proximity proteome reveals Set1-mediated piRNA biogenesis for transposon silencing in telomere
    Article Snippet: Protein samples were denatured at 95°C for 5 min in 2× protein loading buffer [4% (w/v) SDS, 200 mM DTT, 0.1% (v/v) bromophenol blue (BPB), and 20% (v/v) glycerol] saturated with biotin, resolved by SDS–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.2-μm polyvinylidene difluoride membrane (Wako) using the semi-dry system (Trans-blot Turbo, Bio-Rad). .. The membrane was blocked in 4% (w/v) skim milk (Nacalai) in 1× phosphate-buffered saline (PBS) supplemented with 0.1% (v/v) Tween 20 and further incubated with primary antibodies: rabbit anti-GFP (1:1000, Clonetech), mouse anti-Piwi (1:100) ( Saito et al , 2006 ), guinea pig anti-Aub (1:1000) and rat anti-Ago3 (1:200) ( Lim et al , 2022 ), mouse anti-Tubulin (1:3000, Santa Cruz). .. Secondary antibodies were anti-guinea pig immunoglobulins-HRP (1:1000; Dako), anti-rabbit immunoglobulin G (IgG)–HRP (1:3000; Bio-Rad), anti-mouse IgG-HRP (1:3000; Bio-Rad), and anti-rat IgG (1:1000; DAKO).

    other:

    Article Title: Proximity labeling reveals non-catalytic interactions between DPP9 and ubiquitin signaling complexes
    Article Snippet: mouse anti-Tubulin , Santa Cruz , sc-32293.

    Membrane:

    Article Title: PIWI proximity proteome reveals Set1-mediated piRNA biogenesis for transposon silencing in telomere
    Article Snippet: Protein samples were denatured at 95°C for 5 min in 2× protein loading buffer [4% (w/v) SDS, 200 mM DTT, 0.1% (v/v) bromophenol blue (BPB), and 20% (v/v) glycerol] saturated with biotin, resolved by SDS–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.2-μm polyvinylidene difluoride membrane (Wako) using the semi-dry system (Trans-blot Turbo, Bio-Rad). .. The membrane was blocked in 4% (w/v) skim milk (Nacalai) in 1× phosphate-buffered saline (PBS) supplemented with 0.1% (v/v) Tween 20 and further incubated with primary antibodies: rabbit anti-GFP (1:1000, Clonetech), mouse anti-Piwi (1:100) ( Saito et al , 2006 ), guinea pig anti-Aub (1:1000) and rat anti-Ago3 (1:200) ( Lim et al , 2022 ), mouse anti-Tubulin (1:3000, Santa Cruz). .. Secondary antibodies were anti-guinea pig immunoglobulins-HRP (1:1000; Dako), anti-rabbit immunoglobulin G (IgG)–HRP (1:3000; Bio-Rad), anti-mouse IgG-HRP (1:3000; Bio-Rad), and anti-rat IgG (1:1000; DAKO).

    Saline:

    Article Title: PIWI proximity proteome reveals Set1-mediated piRNA biogenesis for transposon silencing in telomere
    Article Snippet: Protein samples were denatured at 95°C for 5 min in 2× protein loading buffer [4% (w/v) SDS, 200 mM DTT, 0.1% (v/v) bromophenol blue (BPB), and 20% (v/v) glycerol] saturated with biotin, resolved by SDS–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.2-μm polyvinylidene difluoride membrane (Wako) using the semi-dry system (Trans-blot Turbo, Bio-Rad). .. The membrane was blocked in 4% (w/v) skim milk (Nacalai) in 1× phosphate-buffered saline (PBS) supplemented with 0.1% (v/v) Tween 20 and further incubated with primary antibodies: rabbit anti-GFP (1:1000, Clonetech), mouse anti-Piwi (1:100) ( Saito et al , 2006 ), guinea pig anti-Aub (1:1000) and rat anti-Ago3 (1:200) ( Lim et al , 2022 ), mouse anti-Tubulin (1:3000, Santa Cruz). .. Secondary antibodies were anti-guinea pig immunoglobulins-HRP (1:1000; Dako), anti-rabbit immunoglobulin G (IgG)–HRP (1:3000; Bio-Rad), anti-mouse IgG-HRP (1:3000; Bio-Rad), and anti-rat IgG (1:1000; DAKO).



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    Image Search Results


    SMFs enhance orthodontic mechano‐transduction via activation in microtubule‐integrin assembly of PDLCs. (a) Heat map for cluster analysis of differentially expressed genes (DEGs). (b) Bubble plot of GO enrichment results of DEGs. (c) Bubble plot of KEGG enrichment results of DEGs. (d) Relative FPKM values of ITGB1, ITGB7, ITGB9, CCL7, TNF and IL6 from RNA‐seq data. Mean ± SD. n = 3. ** p < 0.01, *** p < 0.001. (e) 12h and 24h Relative gene expression of ITGB1, ITGB2, ITGB3, IFNG, MCSF and TNF from qRT‐PCR. Mean ± SD. n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (f) Representative immunofluorescence images of α‐Tubulin (green), Integrin‐β1 (red) (merged yellow) and nuclei (blue) in mechanical force applied PDLCs without and with SMFs. Scale bar: 10 µm. (g) Pearson's coefficient and Manders’ overlap coefficient (M1) for the colocalization analysis between α‐Tubulin and Integrin‐β1. Mean ± SD. n = 30. **** p < 0.0001.

    Journal: Advanced Science

    Article Title: Arrayed Magnetic Accelerator Synergistically Enhances Orthodontic Tooth Movement via Microtubule Assembly‐Mediated Periodontal Remodeling

    doi: 10.1002/advs.77447

    Figure Lengend Snippet: SMFs enhance orthodontic mechano‐transduction via activation in microtubule‐integrin assembly of PDLCs. (a) Heat map for cluster analysis of differentially expressed genes (DEGs). (b) Bubble plot of GO enrichment results of DEGs. (c) Bubble plot of KEGG enrichment results of DEGs. (d) Relative FPKM values of ITGB1, ITGB7, ITGB9, CCL7, TNF and IL6 from RNA‐seq data. Mean ± SD. n = 3. ** p < 0.01, *** p < 0.001. (e) 12h and 24h Relative gene expression of ITGB1, ITGB2, ITGB3, IFNG, MCSF and TNF from qRT‐PCR. Mean ± SD. n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (f) Representative immunofluorescence images of α‐Tubulin (green), Integrin‐β1 (red) (merged yellow) and nuclei (blue) in mechanical force applied PDLCs without and with SMFs. Scale bar: 10 µm. (g) Pearson's coefficient and Manders’ overlap coefficient (M1) for the colocalization analysis between α‐Tubulin and Integrin‐β1. Mean ± SD. n = 30. **** p < 0.0001.

    Article Snippet: PDLCs were respectively incubated with the primary antibody for Vinculin (Proteintech, 26520‐1‐Ap, 1:300), FAK (Proteintech, 12636‐1‐AP, 1:400), YAP/TAZ (Proteintech, 13584‐1‐Ap, 1:200), α‐Tubulin (Abclonal, AC012, 1:200) and Integrin‐β1 (Abclonal, A23497, 1:200).

    Techniques: Transduction, Activation Assay, Analysis, RNA Sequencing, Gene Expression, Quantitative RT-PCR, Immunofluorescence

    Top panel) Western blot showing non-specific bands detected by the anti-DJ-1 antibody NB300-270 in C. elegans. Whole worm lysates from djr-1.2 ( tm1346 ) , djr-1.2 ( tm951 ), and djr-1.1 ( tm918 ) exhibit bands identical to those observed in wild-type ( N2 ) whole worm lysates. Bottom panel) Housekeeping protein α-tubulin demonstrates equal loading of samples. The image shown is representative of three independent experiments.

    Journal: microPublication Biology

    Article Title: A commercially available DJ-1 antibody does not detect C. elegans DJR-1.1 or DJR-1.2 proteins in whole worm lysates

    doi: 10.17912/micropub.biology.002375

    Figure Lengend Snippet: Top panel) Western blot showing non-specific bands detected by the anti-DJ-1 antibody NB300-270 in C. elegans. Whole worm lysates from djr-1.2 ( tm1346 ) , djr-1.2 ( tm951 ), and djr-1.1 ( tm918 ) exhibit bands identical to those observed in wild-type ( N2 ) whole worm lysates. Bottom panel) Housekeeping protein α-tubulin demonstrates equal loading of samples. The image shown is representative of three independent experiments.

    Article Snippet: The membranes were blocked using a blocking buffer (1× Tris-buffered saline, 0.5% Fish Gelatin, 0.1% Tween 20, 0.02% Sodium Azide) then incubated with rabbit anti-DJ-1 antibody (1:1000; Novus Biologicals, NB300-270) overnight at 4°C or a mouse anti-alpha tubulin antibody (1:200; Santa Cruz Biotechnology, SC-32293) at room temperature for several hours.

    Techniques: Western Blot

    Journal: microPublication Biology

    Article Title: A commercially available DJ-1 antibody does not detect C. elegans DJR-1.1 or DJR-1.2 proteins in whole worm lysates

    doi: 10.17912/micropub.biology.002375

    Figure Lengend Snippet:

    Article Snippet: The membranes were blocked using a blocking buffer (1× Tris-buffered saline, 0.5% Fish Gelatin, 0.1% Tween 20, 0.02% Sodium Azide) then incubated with rabbit anti-DJ-1 antibody (1:1000; Novus Biologicals, NB300-270) overnight at 4°C or a mouse anti-alpha tubulin antibody (1:200; Santa Cruz Biotechnology, SC-32293) at room temperature for several hours.

    Techniques: