mouse monoclonal santacruz biotechnology sc (Santa Cruz Biotechnology)
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Mouse Monoclonal Santacruz Biotechnology Sc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 8652 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+tubulin/%CE%B1+Tubulin+Antibody/pm42814109-32-50-52
Average 97 stars, based on 8652 article reviews
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Western Blot:Article Title: Interfacial Coordination-Engineered Emulgel Modulates the Osteo-Immune Microenvironment in Periodontitis Treatment Via HIF1 Signaling Pathway-Induced Metabolic Reprogramming. Article Snippet: .. Antibodies used in western blot as follows: mouse anti-MYD88 antibody (1:2000, 67969-1-Ig, Proteintech, China), mouse anti-mTOR antibody (1:25 000, 66888-1-Ig, Proteintech, China), rabbit anti-AMPK antibody, rabbit anti-HIF-1α(1:1000, ab207442, Abcam, USA), mouse anti-NF-kB (1:1000, #Q04206, Cell Signaling Technology, USA), mouse anti-TNF-α (Proteintech, 60291-1- Ig, 1:1000), rabbit anti-iNOS (1:200, 22226-1-AP, Proteintech, China), mouse anti-NLRP3 (1:2000, 68102-1-lg, Proteintech, China), rabbit antiIL-1β (1:1000, ab254360, Abcam), Incubation:Article Title: Interfacial Coordination-Engineered Emulgel Modulates the Osteo-Immune Microenvironment in Periodontitis Treatment Via HIF1 Signaling Pathway-Induced Metabolic Reprogramming. Article Snippet: .. Antibodies used in western blot as follows: mouse anti-MYD88 antibody (1:2000, 67969-1-Ig, Proteintech, China), mouse anti-mTOR antibody (1:25 000, 66888-1-Ig, Proteintech, China), rabbit anti-AMPK antibody, rabbit anti-HIF-1α(1:1000, ab207442, Abcam, USA), mouse anti-NF-kB (1:1000, #Q04206, Cell Signaling Technology, USA), mouse anti-TNF-α (Proteintech, 60291-1- Ig, 1:1000), rabbit anti-iNOS (1:200, 22226-1-AP, Proteintech, China), mouse anti-NLRP3 (1:2000, 68102-1-lg, Proteintech, China), rabbit antiIL-1β (1:1000, ab254360, Abcam), Article Title: Functional characterization of DPP4 and FcRn as receptor and coreceptor for classical human astroviruses in Caco-2 cells Article Snippet: Cellular lysates of siRNA-transfected cells or cell lysates of untransfected WT, FcRn-KO, DPP4-KO, or double FcRn/DPP4-KO Caco-2 cells were separated by 10% SDS-PAGE; the proteins were then transferred to nitrocellulose membranes (Millipore #HATF00010). .. The membranes were blocked with 5% nonfat milk for 1 h at RT, followed by incubation with either rabbit anti-FcRn (Abcam #ab228975) or anti-DPP4 (Cell Signaling #67138) (dil. 1:1,000) polyclonal antibodies or with Article Title: PIWI proximity proteome reveals Set1-mediated piRNA biogenesis for transposon silencing in telomere Article Snippet: Protein samples were denatured at 95°C for 5 min in 2× protein loading buffer [4% (w/v) SDS, 200 mM DTT, 0.1% (v/v) bromophenol blue (BPB), and 20% (v/v) glycerol] saturated with biotin, resolved by SDS–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.2-μm polyvinylidene difluoride membrane (Wako) using the semi-dry system (Trans-blot Turbo, Bio-Rad). .. The membrane was blocked in 4% (w/v) skim milk (Nacalai) in 1× phosphate-buffered saline (PBS) supplemented with 0.1% (v/v) Tween 20 and further incubated with primary antibodies: rabbit anti-GFP (1:1000, Clonetech), mouse anti-Piwi (1:100) ( Saito et al , 2006 ), guinea pig anti-Aub (1:1000) and rat anti-Ago3 (1:200) ( Lim et al , 2022 ), other:Article Title: Proximity labeling reveals non-catalytic interactions between DPP9 and ubiquitin signaling complexes Article Snippet: Membrane:Article Title: PIWI proximity proteome reveals Set1-mediated piRNA biogenesis for transposon silencing in telomere Article Snippet: Protein samples were denatured at 95°C for 5 min in 2× protein loading buffer [4% (w/v) SDS, 200 mM DTT, 0.1% (v/v) bromophenol blue (BPB), and 20% (v/v) glycerol] saturated with biotin, resolved by SDS–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.2-μm polyvinylidene difluoride membrane (Wako) using the semi-dry system (Trans-blot Turbo, Bio-Rad). .. The membrane was blocked in 4% (w/v) skim milk (Nacalai) in 1× phosphate-buffered saline (PBS) supplemented with 0.1% (v/v) Tween 20 and further incubated with primary antibodies: rabbit anti-GFP (1:1000, Clonetech), mouse anti-Piwi (1:100) ( Saito et al , 2006 ), guinea pig anti-Aub (1:1000) and rat anti-Ago3 (1:200) ( Lim et al , 2022 ), Saline:Article Title: PIWI proximity proteome reveals Set1-mediated piRNA biogenesis for transposon silencing in telomere Article Snippet: Protein samples were denatured at 95°C for 5 min in 2× protein loading buffer [4% (w/v) SDS, 200 mM DTT, 0.1% (v/v) bromophenol blue (BPB), and 20% (v/v) glycerol] saturated with biotin, resolved by SDS–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.2-μm polyvinylidene difluoride membrane (Wako) using the semi-dry system (Trans-blot Turbo, Bio-Rad). .. The membrane was blocked in 4% (w/v) skim milk (Nacalai) in 1× phosphate-buffered saline (PBS) supplemented with 0.1% (v/v) Tween 20 and further incubated with primary antibodies: rabbit anti-GFP (1:1000, Clonetech), mouse anti-Piwi (1:100) ( Saito et al , 2006 ), guinea pig anti-Aub (1:1000) and rat anti-Ago3 (1:200) ( Lim et al , 2022 ), |

